Ionic trapping and even concentration is a well known trick to steer agents in terms of ADME & distribution. IIRC, nicotine accumulates in your stomach regardless of whether you smoke it, chew it or absorb it through the skin. It becomes charged at stomach pH.
Thank you for writing in ways that even biology flunkees like myself can almost understand! I appreciate that effort, and I hope that will translate into people who ARE knowledgeable being able to favorably contribute to this amazing effort to pull back the sheets on this concoction.
Robyn Cosford , I agree and add Dr. Yeadon ‘s comment = truth . For degradation purposes, Autophagy and Berberine and antioxidants ect ... these mechanisms aid in ridding our bodies of this spike via carbohydrate and fat metabolism. FLCCC and Walter Chesnutt stack are right on ! Thanks Jessica for the insight into the devil’s brew .
Your breakdown of the chemistry seems spot on, but it's a myth that chemistry is difficult actually. In any case, consider yourself an honorary chemist!
It seems they don't even know exactly how ionizable lipids interact with cell membranes:
"3.1.4. Proposed mechanism by which LNPs mediate endosomal disruption
Generally, cells are expected to take up LNPs via endocytosis [21]. As endosomes mature, pH decreases to values below the apparent pKa value of the ionizable lipids, a high proportion of which are consequently protonated. It has been suggested that the electrostatic interactions between the cationic ionizable lipids in the LNPs and the anionic lipids in the endosomal membrane lead to a disruption of the endosomal membrane [22]."
Also, it seems that 10% of ionizable lipids might be positively charged in blood:
"When using ionizable lipids/LNPs with an apparent pKa of ∼6.4, about 10% of the ionizable lipids are positively charged in blood (pH 7.4) according to the Henderson–Hasselbalch equation. The surface charge may not only affect the protein corona composition on nanoparticles but also influence the conformation of the adsorbed proteins, including apoE. Taken together, these inquiries show the interplay between nanomedicines, including LNPs and biomolecules such as apoE in biological fluids, is affected by surface composition, surface charge, and particle size [37]. Such interplay determines the in vivo fate of many nanomedicines."
Enough to cause significant toxic effects I would imagine - especially as they were not supposed to be circulating in blood!
Finally, it seems the LNPs used by Moderna and Pfizer were originally optimized for entry to the liver:
"The team made about 100 ionizable lipids and introduced ester linkages into the carbon chains of the lipids to help make them more biodegradable, he recalls. Tweaking the ratio of the four lipids in the nanoparticles altered the LNPs’ distribution in the body. “The devil is absolutely in the details as far as LNPs are concerned,” Ciaramella says. “But once you optimize it for one organ, you can change out the mRNA with minimal optimization.”
"Those ionizable lipids, which are remarkably similar in structure, were discovered while the firms were optimizing LNPs for systemic administration and delivery to the liver—not the intramuscular injection of a vaccine."
Feb 20, 2023·edited Feb 20, 2023Liked by Jessica Rose
I will look in my emails for the webinar name/company but "I'm listening to a Tech webinar on quality control for mRNA vaccines. Very positive and upbeat business attitude.
The last one I listened to on how to make LNPs was basically about a gadget with two inputs. Controlling the flow of the outer material input and the mRNA or other material to be contained in the other input was how size of the LNPs is changed. Modifying the flow rate is all that is needed to change the size of the LNPs that form.
"Super easy, so nonpartisan, can be used for whatever is intended to be contained, not just mRNA." Very upbeat positive business attitude pitching the gadget. The webinar process accepted no public chat comments." A Telegram conversation of mine.
low pH also an issue with increased spike attraction; it seems we are back with the old naturopathic concept of keeping the body alkalinised as far as possible...
apparently 5G as a side effect acidifies the system
I know that cationic surfactants are a lot more toxic than anionic surfactants. That's why we never used them in bioremediation studies to remove chlorinated solvents from soils.
When I read that Pharma was using cationic particles with PEG in their shots, I was shocked.
I will never knowingly allow or offer up my body to these type of injections.
I am retired but after so many years of education I could not stop reading. “This might be on the exam!” What are catatonic lipids and who is Esther? You answered my questions thoroughly. My next thought is: what would Stefanie Seneff say? I listened to a number of her presentations on molecular level poisoning and conclude (1) this research and understanding is important and (2) don’t inject this stuff into humans (or eat it). Same for shots, in general. It is a bitter irony that so much effort (with good reason) goes into detailed study of the damage mechanisms of stuff that does not belong in one’s blood stream to begin with. It can be interesting. We are in a stage of battling mandated medical
Interventions where knowing how the government murders citizens by throwing proverbial “wrenches in the gears” and attempting to repair it with a sledge hammer is a good metaphor for hospital protocols that exterminated 1M Americans. And it WAS NOT the virus, no matter what Fauci says.
thank you Dr Jess and also all of the commenters , as a long time listener to Gary Null it took about two or three days after the crapola hit the fan blades in March 2020 to see this was headed toward a massive jabfest from pHARMa , whose funding of the media to the tune of 70% is how we got here today. the ads need to be banned like cigarettes were and the companies need to be broken up.
I will not stop digging. And whatever I find I will blab to everyone who wants to hear.
Ionic trapping and even concentration is a well known trick to steer agents in terms of ADME & distribution. IIRC, nicotine accumulates in your stomach regardless of whether you smoke it, chew it or absorb it through the skin. It becomes charged at stomach pH.
https://www.bigmarker.com/labx-media-group/Level-Up-Your-LNP-Making-Skills-With-Nunchuck?show_live_page=true&add_to_calendar=true&bmid=d9eb04d187db
HHAHAHAHA it's almost too much for me. UNAGI. https://www.youtube.com/watch?v=0ev_ob4mVwg
I'll keep this short.
Thank you for writing in ways that even biology flunkees like myself can almost understand! I appreciate that effort, and I hope that will translate into people who ARE knowledgeable being able to favorably contribute to this amazing effort to pull back the sheets on this concoction.
Thank you, Jessica!
Robyn Cosford , I agree and add Dr. Yeadon ‘s comment = truth . For degradation purposes, Autophagy and Berberine and antioxidants ect ... these mechanisms aid in ridding our bodies of this spike via carbohydrate and fat metabolism. FLCCC and Walter Chesnutt stack are right on ! Thanks Jessica for the insight into the devil’s brew .
Your breakdown of the chemistry seems spot on, but it's a myth that chemistry is difficult actually. In any case, consider yourself an honorary chemist!
(this coming from a former chemist)
It seems they don't even know exactly how ionizable lipids interact with cell membranes:
"3.1.4. Proposed mechanism by which LNPs mediate endosomal disruption
Generally, cells are expected to take up LNPs via endocytosis [21]. As endosomes mature, pH decreases to values below the apparent pKa value of the ionizable lipids, a high proportion of which are consequently protonated. It has been suggested that the electrostatic interactions between the cationic ionizable lipids in the LNPs and the anionic lipids in the endosomal membrane lead to a disruption of the endosomal membrane [22]."
Also, it seems that 10% of ionizable lipids might be positively charged in blood:
"When using ionizable lipids/LNPs with an apparent pKa of ∼6.4, about 10% of the ionizable lipids are positively charged in blood (pH 7.4) according to the Henderson–Hasselbalch equation. The surface charge may not only affect the protein corona composition on nanoparticles but also influence the conformation of the adsorbed proteins, including apoE. Taken together, these inquiries show the interplay between nanomedicines, including LNPs and biomolecules such as apoE in biological fluids, is affected by surface composition, surface charge, and particle size [37]. Such interplay determines the in vivo fate of many nanomedicines."
Enough to cause significant toxic effects I would imagine - especially as they were not supposed to be circulating in blood!
https://www.sciencedirect.com/science/article/pii/S0169409X22003064
Finally, it seems the LNPs used by Moderna and Pfizer were originally optimized for entry to the liver:
"The team made about 100 ionizable lipids and introduced ester linkages into the carbon chains of the lipids to help make them more biodegradable, he recalls. Tweaking the ratio of the four lipids in the nanoparticles altered the LNPs’ distribution in the body. “The devil is absolutely in the details as far as LNPs are concerned,” Ciaramella says. “But once you optimize it for one organ, you can change out the mRNA with minimal optimization.”
"Those ionizable lipids, which are remarkably similar in structure, were discovered while the firms were optimizing LNPs for systemic administration and delivery to the liver—not the intramuscular injection of a vaccine."
https://cen.acs.org/pharmaceuticals/drug-delivery/Without-lipid-shells-mRNA-vaccines/99/i8
But I'm sure they tweaked them just right.
Hi
Another try here. ...The Covid-19 and the vaccine hoaxes can be precisely lighted on by the strategic-mathematical method:
https://zenodo.org/record/7372672
Fortunately this major repository is based in Switzerland and its editors do not try to censor the content if they "do not like the results"
Dear Jessica, totally fascinating. Every detail can show up a danger.
"Granted, it is hard to convince me of things that weren’t designed by nature". Something we share 100%.
I will look in my emails for the webinar name/company but "I'm listening to a Tech webinar on quality control for mRNA vaccines. Very positive and upbeat business attitude.
The last one I listened to on how to make LNPs was basically about a gadget with two inputs. Controlling the flow of the outer material input and the mRNA or other material to be contained in the other input was how size of the LNPs is changed. Modifying the flow rate is all that is needed to change the size of the LNPs that form.
"Super easy, so nonpartisan, can be used for whatever is intended to be contained, not just mRNA." Very upbeat positive business attitude pitching the gadget. The webinar process accepted no public chat comments." A Telegram conversation of mine.
Here is the Webinar, it is viewable I think. https://www.bigmarker.com/labx-media-group/Level-Up-Your-LNP-Making-Skills-With-Nunchuck?show_live_page=true&add_to_calendar=true&bmid=d9eb04d187db
low pH also an issue with increased spike attraction; it seems we are back with the old naturopathic concept of keeping the body alkalinised as far as possible...
apparently 5G as a side effect acidifies the system
Lipid 5, probably the 5th spot on a thin-layer chromatography plate.
I know that cationic surfactants are a lot more toxic than anionic surfactants. That's why we never used them in bioremediation studies to remove chlorinated solvents from soils.
When I read that Pharma was using cationic particles with PEG in their shots, I was shocked.
I will never knowingly allow or offer up my body to these type of injections.
I am retired but after so many years of education I could not stop reading. “This might be on the exam!” What are catatonic lipids and who is Esther? You answered my questions thoroughly. My next thought is: what would Stefanie Seneff say? I listened to a number of her presentations on molecular level poisoning and conclude (1) this research and understanding is important and (2) don’t inject this stuff into humans (or eat it). Same for shots, in general. It is a bitter irony that so much effort (with good reason) goes into detailed study of the damage mechanisms of stuff that does not belong in one’s blood stream to begin with. It can be interesting. We are in a stage of battling mandated medical
Interventions where knowing how the government murders citizens by throwing proverbial “wrenches in the gears” and attempting to repair it with a sledge hammer is a good metaphor for hospital protocols that exterminated 1M Americans. And it WAS NOT the virus, no matter what Fauci says.
💪🏻💪🏻👊
thank you Dr Jess and also all of the commenters , as a long time listener to Gary Null it took about two or three days after the crapola hit the fan blades in March 2020 to see this was headed toward a massive jabfest from pHARMa , whose funding of the media to the tune of 70% is how we got here today. the ads need to be banned like cigarettes were and the companies need to be broken up.